potential sources of starch-degrading bacteria

Post-Lab Questions

1. Why is cow manure used as a potential source of starch-degrading bacteria? (If you are not familiar with the process of digestion in cows, use a reputable internet source to inform your answer.)

2. What are some other potential sources of starch-degrading bacteria?

Cow dung have a fecal composition that contains lower amounts of protein and a higher percentage of starch. cows fibrous diet dictates a lower need for amylase in the small intestine. The microorganisms found in the cattle manure will harvest the starch as their main source of food instead of protein. so, the cow dung acts as a potential source of starch degrading bacteria.

3. What component makes starch agar selective for starch-degrading bacteria?

Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper

starch-degrading bacteria in pure culture from soil using a starch laden agar medium

The two bacterial strains Bacillus amyloliquefaciens and Bacillus licheniformis

Cow manure

Millions of enzymes, each with a specific role, are required in nature to break down compounds during the decomposition process (and make compounds in catabolic processes). By purifying these enzymes and producing them on a large scale, they can be used for a vast range of applications in the service of mankind. Humans have used bacteria and the enzymes they produce for thousands of years (e.g. cheese making). Common exploitation of microorganisms for decomposition of substances include use of bacteria in sewage treatment plants and in composting. On a more experimental basis microbes have been used to decompose contamination from oil spills and other hazardous contaminants in ground water (bioremediation). Almost all enzymes used in these applications originate from microorganisms found in the soil

4. Why were each of the following steps performed in this experiment?

a. Serial dilution: Serial dilution: It is normally done if the chemical concentration is too high than the desired composition. It is used to diminish the thick culture to a more usable concentration.

b. Growth on the nutrient agar plates: Growth on the nutrient agar plates: Growth on the nutrient agar plates is carried out for isolation and purification of cultures. This is used to produce the bacterial lawns needed for the tests. When the growth appears on the agar we are able to perform our startch degrading bacterial experiments. Therefore, this is used for isolation and purification of culture ultimately.

c. Streak on the starch agar plates: Streak on the starch agar plates: Streak on the starch agar plates is a qualitative isolation method in order to isolate and sample individual bacterial colonies.

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